Corning® Epidermal Growth Factor (EGF), Human Recombinant, 100 µg

Corning® Epidermal Growth Factor (EGF), human recombinant, 100µg, is a low-molecular-weight mitogenic protein that stimulates proliferation of a wide variety of cell types in vitro. 1/pack. These growth factors have been used to promote long-term proliferation in cell culture and to induce cell differentiation in a variety of cell types under serum-free or reduced serum conditions.

Formulation

Lyophilized from acetic acid, carrier-free; reconstitute in dH2O or aqueous buffer

Preparation and Storage

Stable for at least three months at 2° to 8°C (lyophilized) or = three months at -20°C (solubilized). Do not store in frost-free freezer. Avoid multiple freeze/thaws.

Quality

• Purity: >98% by SDS-PAGE and reverse phase HPLC
• Shown to stimulate proliferation of human foreskin fibroblasts in serum-containing medium
• Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma

Source

Recombinant, E. coli

Molecular Weight

6.2 kD

Tip

Recommended concentration: 0.1 to 1.0 ng/ mL medium depending on cell type