GLUTATHIONE SEPHAROSE HP 25 ML

Glutathione Sepharose High Performance, 25 ml
Glutathione Sepharose High Performance is an affinity medium for high resolution purification of Glutathione S transferase (GST) tagged proteins.
34 µm bead size for improved efficiency and resolution
Fast, one-step purification of GST-tagged proteins and other glutathione S-trasferase or glutathione-dependent proteins
Mild conditions during the purification process preserves protein antigenicity and function
Glutathione Sepharose High Performance is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function


Features:
•34 µm bead size for improved efficiency and resolution
•Fast, one-step purification of GST-tagged proteins and other glutathione S-trasferase or glutathione-dependent proteins
•Mild conditions during the purification process preserves protein antigenicity and function

Glutathione Sepharose High Performance is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function

Binding Capacity/ml Chromatography Medium	>Approx. 10 mg GST-tagged protein/ml medium Binding capacity will vary depending of the type of cell lysate, target protein, flow rate, temperature, pH, etc. This is an important consideration, especially during sample loading and elution.
Chemical Stability	All commonly used aqueous buffers
Complete Packsize	25 ml
Flow Velocity	<600 cm/h
Ligand	Glutathione
Matrix	Highly crossed-linked agarose, 6%
pH Stability Cleaning-in-Place (CIP)	3–12
pH Stability Working Range	3–12
Storage Conditions	4 to 30°C, 20% Ethanol
Particle size, d50V	34 μm d50v is the median particle size of the cumulative volume distribution.
pH stability, CIP	3–12