NI SEPHAROSE HP 25 ML

Ni Sepharose High Performance, 25 ml
Ni Sepharose High Performance affinity media for high-resolution, high capacity purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
High-performance purification of histidine-tagged proteins
Negligible leakage of Ni²⁺ ions.
High binding capacity, at least 40 mg/ml medium
Compatible with a wide range of reducing agents, detergents, denaturants and other additives
34 µm bead size for improved resolution
Ni Sepharose High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni2+, which will selectively retain proteins with exposed histidine groups.
The high stability and broad compatibility of Ni Sepharose High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions


Features:
•High-performance purification of histidine-tagged proteins
•Negligible leakage of Ni²⁺ ions.
•High binding capacity, at least 40 mg/ml medium
•Compatible with a wide range of reducing agents, detergents, denaturants and other additives
•34 µm bead size for improved resolution

Ni Sepharose High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni2+, which will selectively retain proteins with exposed histidine groups.

The high stability and broad compatibility of Ni Sepharose High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions

Avoid Using	Chelating agents such as EDTA, EGTA , citrate
Binding Capacity/ml Chromatography Medium	At least 40 mg histidine-tagged protein/ml medium Protein binding capacity is protein-to-protein dependent.
Chemical Stability	For Ni2+-stripped medium 0.01 M HCl, 0.1 M NaOH. Tested for one week at 40°C 1 M NaOH, 70% HAc. Tested for 12 h 2% SDS. Tested for 1 h 30% 2-propanol. Tested for 30 min
Complete Packsize	25 ml
Matrix	Highly cross-linked agarose, 6%
Metal Ion Capacity	approx. 15 µmol Ni2+/ml medium
pH Stability Cleaning-in-Place (CIP)	2–14 Ni²?-stripped medium.
pH Stability Working Range	3–12 Ni²?-stripped medium.
Storage Conditions	4 to 30°C, 20% Ethanol
Particle size, d50V	34 µm d50v is the median particle size of the cumulative volume distribution.
pH stability, CIP	2–14 Ni²?-stripped medium.