ADAR1, Human Recombinant Protein
Ordering Info:Product Number: P002H.1
Product Type: Active recombinant protein
Function: Hydrolytic deamination of adenosine to inosine in double-stranded (ds) RNA (A-to-I editing)
Synonyms: ADAR, IFI-4, IFI4, P136, DRADA, SDRAD, DSH, AGS6, G1P1, K88DSRBP
UniProt: P55265
NCBI: NP_001020278.1, NM_001025107.3
Organism: Human
Host: Saccharomyces cerevisiae
Tagging: N-terminal MBP
Region: 701 – 1226 (dsRNA binding domain #3 + deaminase domain)
Sequence: SLDNLESMMPNKVRKIGELVRYLNTNPVGGLLEYARSHGFAAEFKLVDQSGPPHEPKFVYQAKVGGRWFPAVCA
HSKKQGKQEAADAALRVLIGENEKAERMGFTEVTPVTGASLRRTMLLLSRSPEAQPKTLPLTGSTFHDQIAMLSHR
CFNTLTNSFQPSLLGRKILAAIIMKKDSEDMGVVVSLGTGNRCVKGDSLSLKGETVNDCHAEIISRRGFIRFLYSELM
KYNSQTAKDSIFEPAKGGEKLQIKKTVSFHLYISTAPCGDGALFDKSCSDRAMESTESRHYPVFENPKQGKLRTKVE
NGEGTIPVESSDIVPTWDGIRLGERLRTMSCSDKILRWNVLGLQGALLTHFLQPIYLKSVTLGYLFSQGHLTRAICCR
VTRDGSAFEDGLRHPFIVNHPKVGRVSIYDSKRQSGKTKETSVNWCLADGYDLEILDGTRGTVDGPRNELSRVSKK
NIFLLFKKLCSFRYRRDLLRLSYGEAKKAARDYETAKNYFKKGLKDMGYGNWISKPQEEKNFYLCPV
Calculated MW: 101.7 kDa (including tag)
Preparation: three-stage chromatographic purification
Purification grade: crystallography
Purity: >95% (as determined by SDS PAGE and Size Exclusion Chromatography)
Formulation: 20 mM Tris/HCl pH 7.5, 150 mM NaCl, 10 mM βME, 20% glycerol
Activity check: the enzyme activity has been validated in dsRNA editing assay
Storage: -80C
Shipping: shipped on dry ice
Keywords: Excella