Toxin B, C.difficile Recombinant, 100 mg
Item No.
61171194
Toxin B, Recombinant Protein Ordering Info: Part Number Size (mg) 61171193 10 61171194 100 61171195 1000 Product Number: P009B.1 Product Type: Active recombinant protein Function: Catalytic domain of the main toxin of C.difficile infection; disrupts the colonic epithelium resulting in severe
Description
Toxin B, Recombinant Protein
Ordering Info:
Product Number: P009B.1
Product Type: Active recombinant protein
Function: Catalytic domain of the main toxin of C.difficile infection; disrupts the colonic epithelium
resulting in severe diarrhea and pseudomembranous colitis
Synonyms: TcdB, toxB, Glucosyltransferase TcdB
UniProt: P18117
NCBI: WP_009902069.1, NZ_SRKJ01000018.1
Organism: Clostridium difficile
Host: Escherichia coli
Tagging: untagged
Region: 1 – 543 (glucosyltransferase domain)
Sequence: MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVEKYLKLKDINSLTDIYI
DTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVWIGGQINDTAINYINQWKD
VNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFRENLNDPRFDYNKFFRKRMEIIY
DKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEIDELNTYIEESLNKITQNSGNDV
RNFEEFKNGESFNLYEQELVERWNLAAASDILRISALKEIGGMYLDVDMLPGIQPDLFES
IEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEHFDMLDEEVQSSFESVLASKSDKSEIF
SSLGDMEASPLEVKIAFNSKGIINQGLISVKDSYCSNLIVKQIENRYKILNNSLNPAISE
DNDFNTTTNTFIDSIMAEANADNGRFMMELGKYLRVGFFPDVKTTINLSGPEAYAAAYQD
LLMFKEGSMNIHLIEADLRNFEISKTNISQSTEQEMASLWSFDDARAKAQFEEYKRNYFE
GSL
Calculated MW: 63.1 kDa
Preparation: five-stage chromatographic purification
Purification grade: crystallography
Purity: >95% (as determined by SDS PAGE and Size Exclusion Chromatography)
Formulation: 20 mM Tris/HCl pH 7.5, 150 mM NaCl, 20% glycerol
Activity check: active as assayed by its ability to hydrolyze ATP UDP-glucose, using the Transcreener ADP2
fluorescence polarization assay (BellBrook Labs) (Letourneau et al, 2018)
Storage: -80C
Shipping: shipped on dry ice
Keywords: Excella
Ordering Info:
| Part Number | Size (mg) |
|---|---|
| 61171193 | 10 |
| 61171194 | 100 |
| 61171195 | 1000 |
Product Number: P009B.1
Product Type: Active recombinant protein
Function: Catalytic domain of the main toxin of C.difficile infection; disrupts the colonic epithelium
resulting in severe diarrhea and pseudomembranous colitis
Synonyms: TcdB, toxB, Glucosyltransferase TcdB
UniProt: P18117
NCBI: WP_009902069.1, NZ_SRKJ01000018.1
Organism: Clostridium difficile
Host: Escherichia coli
Tagging: untagged
Region: 1 – 543 (glucosyltransferase domain)
Sequence: MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVEKYLKLKDINSLTDIYI
DTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVWIGGQINDTAINYINQWKD
VNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFRENLNDPRFDYNKFFRKRMEIIY
DKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEIDELNTYIEESLNKITQNSGNDV
RNFEEFKNGESFNLYEQELVERWNLAAASDILRISALKEIGGMYLDVDMLPGIQPDLFES
IEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEHFDMLDEEVQSSFESVLASKSDKSEIF
SSLGDMEASPLEVKIAFNSKGIINQGLISVKDSYCSNLIVKQIENRYKILNNSLNPAISE
DNDFNTTTNTFIDSIMAEANADNGRFMMELGKYLRVGFFPDVKTTINLSGPEAYAAAYQD
LLMFKEGSMNIHLIEADLRNFEISKTNISQSTEQEMASLWSFDDARAKAQFEEYKRNYFE
GSL
Calculated MW: 63.1 kDa
Preparation: five-stage chromatographic purification
Purification grade: crystallography
Purity: >95% (as determined by SDS PAGE and Size Exclusion Chromatography)
Formulation: 20 mM Tris/HCl pH 7.5, 150 mM NaCl, 20% glycerol
Activity check: active as assayed by its ability to hydrolyze ATP UDP-glucose, using the Transcreener ADP2
fluorescence polarization assay (BellBrook Labs) (Letourneau et al, 2018)
Storage: -80C
Shipping: shipped on dry ice
Keywords: Excella
Item Info:
| Item Title | Toxin B, C.difficile Recombinant, 100 mg |
| Sales Unit of Measure | EA1 |
| Last Date/Time Modified | 3/20/2024 9:22:35 AM |
