HITRAP Q HP 5X5 ML

HiTrap Q HP, 5 x 5 ml
HiTrap Q HP are prepacked, ready-to-use Q Sepharose High Performance strong anion exchange columns for high-resolution, small-scale protein purification.
34 µm bead size for high-performance, high-resolution purifications.
Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
Designed for use with a syringe, peristaltic pump, and chromatography systems such as ÄKTA design.
Strong quaternary ammonium (Q) anion exchanger.
The small particle size (34µm) of the medium allows fast adsorption and desorption even at high sample loadings and flow rates.SP Sepharose High Performance is a strong cation exchange medium and Q Sepharose High Performance is a strong anion exchange medium. Both remain charged and have high loading capacities over broad pH ranges.


Features:
•34 µm bead size for high-performance, high-resolution purifications.
•Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
•Designed for use with a syringe, peristaltic pump, and chromatography systems such as ÄKTA design.
•Strong quaternary ammonium (Q) anion exchanger.

The small particle size (34µm) of the medium allows fast adsorption and desorption even at high sample loadings and flow rates.SP Sepharose High Performance is a strong cation exchange medium and Q Sepharose High Performance is a strong anion exchange medium. Both remain charged and have high loading capacities over broad pH ranges.

Bed Dimensions	16 mm × N/A
Bed Dimensions-column	16 mm × N/A
Bed Volume	5
Charged Groups-media	- N+(CH₃)₃
Chemical Stability-media	Stable in all commonly used buffers: 8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH, 1 M acetic acid, 30% isopropanol, 30% acetonitrile, 2% SDS 1 M NaOH and acetic acid should be used only for cleaning purposes.
Column i.d.-column	16
Complete Packsize	5 x 5 ml
Complete Packsize-column	5 ml
Exclusion Limit [Mr] [Globular Proteins]-media	4 x 10⁶
Ion Exchanger Type-media	Strong anion exchanger
Ligand-media	Quaternary amine
Material [Column Hardware]-column	Polypropylene (PP)
Matrix-media	6% cross-linked agarose
pH Stability Cleaning-in-Place (CIP)-media	1–14
pH Stability Working Range-media	2–12
Pressure Max. [Over the Packed Bed During Operation]	N/A (N/A)
Pressure/Flow Specification-media	150 cm/h (at < 3 bar, 25°C, 10 cm bed height)
Storage Conditions	4 to 30°C, 20% Ethanol
Storage Conditions-media	4 to 30°C, 20% Ethanol
BioProcess resin-media	Yes
Particle size, d50V-media	34 µm d50v is the median particle size of the cumulative volume distribution.
pH stability, CIP-media	1–14