LSD1, Human Recombinant, 100 mg lysine-specific demethylase 1

LSD1, Human Recombinant Protein


Ordering Info:

Part Number	Size (mg)
61171196	10
61171197	100
61171198	500

Product Number: P010H.1
Product Type: Active recombinant protein
Function: Modifies histone H3 by demethylating Lys-4 and Lys-9, acting thereby as a coactivator or a
corepressor
Synonyms: KDM1A, KIAA0601, AOF2, BHC110, CPRF
UniProt: O60341
NCBI: NP_001009999.1, NM_001009999.3
Organism: Human
Host: Escherichia coli
Tagging: N-terminal 6xHis
Region: 172-836
Sequence: SGVEGAAFQSRLPHDRMTSQEAACFPDIISGPQQTQKVFLFIRNRTLQLWLDNPKIQLTFEATL
QQLEAPYNSDTVLVHRVHSYLERHGLINFGIYKRIKPLPTKKTGKVIIIGSGVSGLAAARQLQSF
GMDVTLLEARDRVGGRVATFRKGNYVADLGAMVVTGLGGNPMAVVSKQVNMELAKIKQKC
PLYEANGQAVPKEKDEMVEQEFNRLLEATSYLSHQLDFNVLNNKPVSLGQALEVVIQLQE
KHVKDEQIEHWKKIVKTQEELKELLNKMVNLKEKIKELHQQYKEASEVKPPRDITAEFLV
KSKHRDLTALCKEYDELAETQGKLEEKLQELEANPPSDVYLSSRDRQILDWHFANLEFAN
ATPLSTLSLKHWDQDDDFEFTGSHLTVRNGYSCVPVALAEGLDIKLNTAVRQVRYTASGC
EVIAVNTRSTSQTFIYKCDAVLCTLPLGVLKQQPPAVQFVPPLPEWKTSAVQRMGFGNLN
KVVLCFDRVFWDPSVNLFGHVGSTTASRGELFLFWNLYKAPILLALVAGEAAGIMENISD
DVIVGRCLAILKGIFGSSAVPQPKETVVSRWRADPWARGSYSYVAAGSSGNDYDLMAQPI
TPGPSIPGAPQPIPRLFFAGEHTIRNYPATVHGALLSGLREAGRIADQFLGAMYTL
Calculated MW: 74.8 kDa (including tag)
Preparation: five-stage chromatographic purification
Purification grade: crystallography
Purity: >95% (as determined by SDS PAGE and Size Exclusion Chromatography)
Formulation: 20 mM Tris/HCl pH 7.5, 200 mM NaCl, 1 mM DTT, 20% glycerol
Activity check: the enzyme activity has been validated in peroxidase-coupled assay with methylated histone H3
tail peptide (H3K4Me2) (Forneris et al, 2005)
Storage: -80C
Shipping: shipped on dry ice

Keywords: Excella